SYNTHETIC PEPTIDE INFORMATION


Peptide name Legin 9/10/33
Gene ID Medtr3g067540 Medtr3g067555 Medtr3g067550
Family Name Leginsulin (Legin)
Known SSP Family ID Legin
Peptide sequence DKCGAYCPYPRLYCSGDCDCEPFIASLPPRLNFKCVTPHS
Peptide Length 40
Modifications -
Molecular weight (avg) 4468.20
Pl 6.670
Gravy -0.2700000
Company PEPSCAN
Purity % 97.9
Amount (mg) 10.0
Aliquot (mg) 1
Aliquot to 1 mM (uL) 219.1
Solubility Prediction Good water solubility
Reference -


ROOT TRAITS


Root Phenotype Summary Longer primary and lateral roots and somewhat higher lateral root density
Root Phenotype Summary Images: Peptide treated x Control - untreated (no peptide)
Ca-Spike Assay positive



PRIMARY ROOT TRAITS

* Significant phenotypes observed
Mt: Medicago truncatula; At: Arabidopsis thaliana; Pv: Panicum virgatum

Mt Primary root length (cm) 125%*
At Primary root length (cm) -
Pv Primary root length (cm) -
Mt Lateral root density (no/cm) 111%
At Lateral root density (no/cm) -
Pv Lateral root density (no/cm) -
Mt Mean diameter (cm) 102%
At Mean diameter (cm) -
Pv Mean diameter (cm) -
Mt Surface area (cm2) 126%*
At Surface area (cm2) -
Pv Surface area (cm2) -
Mt Volume (cm3) 122%*
At Volume (cm3) -
Pv Volume (cm3) -
Mt Straightness (vector length/total length) 100%
At Straightness (vector length/total length) -
Pv Straightness (vector length/total length) -

LATERAL ROOT TRAITS

* Significant phenotypes observed
Mt: Medicago truncatula; At: Arabidopsis thaliana; Pv: Panicum virgatum

Mt Total number of lateral roots 126%
Mt Total lateral root length (cm) 139%*
At Total lateral root length (cm) -
Mt Number of secondary lateral roots 138%*
At Number of secondary lateral roots -
Pv Number of secondary lateral roots -
Mt Total length of secondary lateral roots 146%*
At Total length of secondary lateral roots -
Pv Total length of secondary lateral roots -
Mt Mean length of secondary lateral roots 101%
At Mean length of secondary lateral roots -
Pv Mean length of secondary lateral roots -
Mt Mean diameter of secondary lateral roots (cm) 104%
At Mean diameter of secondary lateral roots (cm) -
Pv Mean diameter of secondary lateral roots (cm) -
Mt Mean diameter of all lateral roots (cm) 105%
Mt Surface area of secondary lateral roots (cm2) 152%*
At Surface area of secondary lateral roots (cm2) -
Pv Surface area of secondary lateral roots (cm2) -
Mt Volume of secondary lateral roots (cm3) 156%*
At Volume of secondary lateral roots (cm3) -
Pv Volume of secondary lateral roots (cm3) -
Mt Insertion angle of secondary lateral roots -
At Insertion angle of secondary lateral roots -
Pv Insertion angle of secondary lateral roots -

TOTAL ROOT TRAITS

* Significant phenotypes observed
Mt: Medicago truncatula; At: Arabidopsis thaliana; Pv: Panicum virgatum

Mt Total root length (cm) 135%*
At Total root length (cm) -
Pv Total root length (cm) -
Mt Surface area of total root system (cm2) 137%*
At Surface area of total root system (cm2) -
Pv Surface area of total root system (cm2) -
Mt Total root system volume (cm3) 134%*
At Total root system volume (cm3) -
Pv Total root system volume (cm3) -

NODULE TRAITS

* Significant phenotypes observed
Mt: Medicago truncatula

Nodule Phenotype Summary fewer nodules, lower nodule density
Mt Nodule number 15%*
Mt Nodule density 15%*