SYNTHETIC PEPTIDE INFORMATION


Peptide name CIF1
Gene ID Medtr8g037800
Family Name Casparian Strip Integrity Factor (CIF)
Known SSP Family ID CIF
Peptide sequence D(sY)GRYD(Hyp)S(Hyp)TFSKPPFKLIPN
Peptide Length 21
Modifications sY; Hyp; Hyp
Molecular weight (avg) 2551.70
Pl 9.700
Gravy None
Company PEPSCAN
Purity % 90.9
Amount (mg) 10.0
Aliquot (mg) 1
Aliquot to 1 mM (uL) 356.2
Solubility Prediction Good water solubility
Reference -


ROOT TRAITS


Root Phenotype Summary shorter primary root and slightly longer and larger diameter lateral roots
Root Phenotype Summary Images: Peptide treated x Control - untreated (no peptide)
Ca-Spike Assay negative



PRIMARY ROOT TRAITS

* Significant phenotypes observed
Mt: Medicago truncatula; At: Arabidopsis thaliana; Pv: Panicum virgatum

Mt Primary root length (cm) 83%*
At Primary root length (cm) -
Pv Primary root length (cm) -
Mt Lateral root density (no/cm) 96%
At Lateral root density (no/cm) -
Pv Lateral root density (no/cm) -
Mt Mean diameter (cm) 103%
At Mean diameter (cm) -
Pv Mean diameter (cm) -
Mt Surface area (cm2) 85%*
At Surface area (cm2) -
Pv Surface area (cm2) -
Mt Volume (cm3) 87%*
At Volume (cm3) -
Pv Volume (cm3) -
Mt Straightness (vector length/total length) 100%
At Straightness (vector length/total length) -
Pv Straightness (vector length/total length) -

LATERAL ROOT TRAITS

* Significant phenotypes observed
Mt: Medicago truncatula; At: Arabidopsis thaliana; Pv: Panicum virgatum

Mt Total number of lateral roots 98%
Mt Total lateral root length (cm) 98%
At Total lateral root length (cm) -
Mt Number of secondary lateral roots 81%
At Number of secondary lateral roots -
Pv Number of secondary lateral roots -
Mt Total length of secondary lateral roots 90%
At Total length of secondary lateral roots -
Pv Total length of secondary lateral roots -
Mt Mean length of secondary lateral roots 113%
At Mean length of secondary lateral roots -
Pv Mean length of secondary lateral roots -
Mt Mean diameter of secondary lateral roots (cm) 114%*
At Mean diameter of secondary lateral roots (cm) -
Pv Mean diameter of secondary lateral roots (cm) -
Mt Mean diameter of all lateral roots (cm) 112%*
Mt Surface area of secondary lateral roots (cm2) 104%
At Surface area of secondary lateral roots (cm2) -
Pv Surface area of secondary lateral roots (cm2) -
Mt Volume of secondary lateral roots (cm3) 119%
At Volume of secondary lateral roots (cm3) -
Pv Volume of secondary lateral roots (cm3) -
Mt Insertion angle of secondary lateral roots -
At Insertion angle of secondary lateral roots -
Pv Insertion angle of secondary lateral roots -

TOTAL ROOT TRAITS

* Significant phenotypes observed
Mt: Medicago truncatula; At: Arabidopsis thaliana; Pv: Panicum virgatum

Mt Total root length (cm) 93%
At Total root length (cm) -
Pv Total root length (cm) -
Mt Surface area of total root system (cm2) 99%
At Surface area of total root system (cm2) -
Pv Surface area of total root system (cm2) -
Mt Total root system volume (cm3) 101%
At Total root system volume (cm3) -
Pv Total root system volume (cm3) -

NODULE TRAITS

* Significant phenotypes observed
Mt: Medicago truncatula

Nodule Phenotype Summary no statistical difference
Mt Nodule number 45%
Mt Nodule density 45%