• Gene Family Details



FAMILY INFORMATION


Family name LAT52/Pollen Ole e 1 Allergen (LAT52-POE)
Class Cys-rich
Mode of Action Signal
Post-translational modifications (PTMs) 4-6 Cysteines
Receptor -
Evolutionary Range -
Function No biological function have been proposed; perhaps developmental regulators throughout plant tissues
References
  • The major olive pollen allergen (Ole e I) shows both gametophytic and sporophytic expression during anther development, and its synthesis and storage takes place in the RER The major olive pollen allergen (Ole e I) shows both gametophytic and sporophytic expression during anther development, and its synthesis and storage takes place in the RER J Cell Sci . 1999 Aug;112 ( Pt 15):2501-9. Authors J de Dios Alché   1 ,  A J Castro ,  A Olmedilla ,  M C Fernández ,  R Rodríguez ,  M Villalba ,  M I Rodríguez-García Affiliation 1 Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, E-18008 Granada, Spain. PMID: 10393806 Abstract The distribution of Ole e I (the major olive pollen allergen) and its transcripts was investigated in the anther from premeiotic stages until the dehiscent pollen stage. Crude protein extracts were analyzed by immunoblotting and probed with a monoclonal antibody to Ole e I. The protein, with three variants, was found to accumulate from the early microspore stage onwards. In addition to the previously reported localization of the protein, Ole e I has been immunolocalized for the first time within the pollen wall and in the tapetum. Reverse transcription-polymerase chain reaction analysis using specific oligonucleotides and RNA extracted from whole anthers revealed that the Ole e I gene is expressed from the late tetrad stage onwards. No expression was found in control tissues such as petals, roots or leaves. Light microscopy in situ hybridization on developing flower buds and dehiscent pollen confirmed the transcripts to be present in both the microspores and the sporophytic tissue (tapetum). Labeling was found primarily in the tapetum, reaching the highest concentration in the cytoplasm of the developing and mature pollen, once tapetum started to degenerate. In situ hybridization at the transmission electron microscope level showed the transcripts to accumulate on ribosomes of the rough endoplasmic reticulum. These studies, together with others carried out previously by us, indicated that both synthesis and storage of Ole e I take place in the endoplasmic reticulum, coincidentally with the conspicuous changes suffered by this membrane system during pollen development. This process is most likely controlled at the transcriptional level. The localization of the protein in the pollen ectexine bring new insights into the function of the allergen, which are discussed. Publication types Research Support, Non-U.S. Gov't MeSH terms Allergens / genetics* Antigens, Plant Endoplasmic Reticulum, Rough / metabolism* Endoplasmic Reticulum, Rough / ultrastructure Gene Expression Regulation, Developmental Gene Expression Regulation, Plant* Microscopy, Immunoelectron Plant Proteins / biosynthesis Plant Proteins / genetics* Plant Proteins / metabolism* RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic Trees / genetics* Trees / growth & development Substances Allergens Antigens, Plant Ole e I protein, Olea europaea Plant Proteins RNA, Messenger
  • Genome-wide identification and molecular characterization of Ole_e_I, Allerg_1 and Allerg_2 domain-containing pollen-allergen-like genes in Oryza sativa Genome-wide identification and molecular characterization of Ole_e_I, Allerg_1 and Allerg_2 domain-containing pollen-allergen-like genes in Oryza sativa DNA Res . 2005;12(3):167-79.
  • A cysteine-rich extracellular protein, LAT52, interacts with the extracellular domain of the pollen receptor kinase LePRK2 A cysteine-rich extracellular protein, LAT52, interacts with the extracellular domain of the pollen receptor kinase LePRK2 Plant Cell . 2002 Sep;14(9):2277-87.

HMM PROFILE


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GENE MEMBERS DETAILS


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Gene Assembly Chromossome Coordinates SSP Type SSP Family member SignalP D-value Protein Length Annotation
Medtr0390s0010 Mt4.0v1 scaffold0390 2001-2792 known SSP LAT52/POE17 0.928 164 pollen Ole e I family allergens conf_class=HC
Medtr0390s0040 Mt4.0v1 scaffold0390 14985-15776 known SSP LAT52/POE18 0.928 161 pollen Ole e I family allergens conf_class=HC
Medtr0786s0020 Mt4.0v1 scaffold0786 3469-4259 known SSP LAT52/POE19 0.925 164 pollen Ole e I family allergens conf_class=HC
Medtr1678s0010 Mt4.0v1 scaffold1678 275-1031 known SSP LAT52/POE20 0.906 178 pollen protein Ole E I-like protein conf_class=LC
Medtr1g055415 Mt4.0v1 chr1 24539849-24541448 known SSP LAT52/POE34 0.847 343 pollen Ole e I family allergens conf_class=HC
Medtr1g087935 Mt4.0v1 chr1 39370474-39372003 known SSP LAT52/POE4 0.931 447 pollen Ole e I family allergens conf_class=HC
Medtr1g088110 Mt4.0v1 chr1 39374449-39376935 known SSP LAT52/POE5 0.924 582 pollen Ole e I family allergens conf_class=HC
Medtr1g088130 Mt4.0v1 chr1 39382581-39383859 known SSP LAT52/POE6 0.844 135 pollen Ole e I family allergens conf_class=HC
Medtr2g016020 Mt4.0v1 chr2 4817560-4818701 known SSP LAT52/POE21 0.813 168 pollen Ole e I family allergens conf_class=HC
Medtr2g077080 Mt4.0v1 chr2 32240664-32241400 known SSP LAT52/POE22 0.857 165 pollen Ole e I family allergens conf_class=HC
Medtr2g077940 Mt4.0v1 chr2 32295140-32296238 known SSP LAT52/POE23 0.920 174 pollen Ole e I family allergens conf_class=HC
Medtr2g077960 Mt4.0v1 chr2 32302809-32303898 known SSP LAT52/POE24 0.909 168 pollen Ole e I family allergens conf_class=HC
Medtr2g086680 Mt4.0v1 chr2 36446668-36448760 known SSP LAT52/POE7 0.841 172 pollen Ole e I family allergens conf_class=HC
Medtr2g097240 Mt4.0v1 chr2 41522187-41525095 known SSP LAT52/POE33 0.291 341 pollen protein Ole E I-like protein conf_class=LC
Medtr2g436350 Mt4.0v1 chr2 14141062-14141904 known SSP LAT52/POE13 0.789 155 pollen Ole e I family allergens conf_class=HC
Medtr2g450190 Mt4.0v1 chr2 22138472-22139263 known SSP LAT52/POE25 0.928 161 pollen Ole e I family allergens conf_class=HC
Medtr3g012020 Mt4.0v1 chr3 3174857-3176250 known SSP LAT52/POE37 0.863 393 pollen Ole e I family allergens conf_class=HC
Medtr3g051110 Mt4.0v1 chr3 20164912-20168647 known SSP LAT52/POE14 0.835 147 pollen protein Ole E I-like protein conf_class=HC
Medtr3g071470 Mt4.0v1 chr3 32063196-32064085 known SSP LAT52/POE9 0.894 168 pollen Ole e I family allergens conf_class=HC
Medtr3g073990 Mt4.0v1 chr3 33393582-33395731 known SSP LAT52/POE26 0.914 162 pollen Ole e I family allergens conf_class=HC
Medtr3g087540 Mt4.0v1 chr3 39681582-39682315 known SSP LAT52/POE27 0.864 160 pollen Ole e I family allergens conf_class=HC
Medtr3g117760 Mt4.0v1 chr3 55143434-55145943 known SSP LAT52/POE3 0.810 298 pollen Ole e I family allergens conf_class=HC
Medtr4g017240 Mt4.0v1 chr4 5341759-5343041 known SSP LAT52/POE38 0.881 347 pollen Ole e I family allergens conf_class=HC
Medtr4g046767 Mt4.0v1 chr4 16591718-16592799 known SSP LAT52/POE29 0.875 277 pollen Ole e I family allergens conf_class=HC
Medtr4g046780 Mt4.0v1 chr4 16602288-16603352 known SSP LAT52/POE30 0.904 252 pollen Ole e I family allergens conf_class=HC
Medtr4g066680 Mt4.0v1 chr4 25222320-25223534 known SSP LAT52/POE39 0.874 338 pollen Ole e I family allergens conf_class=HC
Medtr4g075333 Mt4.0v1 chr4 28782249-28784971 known SSP LAT52/POE15 0.905 526 pollen protein Ole E I-like protein conf_class=LC
Medtr4g088735 Mt4.0v1 chr4 35325086-35327598 known SSP LAT52/POE35 0.908 276 pollen Ole e I family allergens conf_class=HC
Medtr4g088740 Mt4.0v1 chr4 35334462-35335943 known SSP LAT52/POE40 0.879 402 pollen Ole e I family allergens conf_class=HC
Medtr4g124400 Mt4.0v1 chr4 51457430-51458050 known SSP LAT52/POE41 0.872 206 pollen Ole e I family allergens conf_class=HC
Medtr4g133932 Mt4.0v1 chr4 56057651-56060052 known SSP LAT52/POE31 0.946 206 pollen Ole e I family allergens conf_class=HC
Medtr5g014340 Mt4.0v1 chr5 4803323-4805515 known SSP LAT52/POE10 0.138 245 pollen protein Ole E I-like protein conf_class=LC
Medtr5g047890 Mt4.0v1 chr5 20963820-20965177 known SSP LAT52/POE42 0.760 342 pollen Ole e I family allergens conf_class=HC
Medtr6g015995 Mt4.0v1 chr6 5684113-5686084 known SSP LAT52/POE1 0.758 346 pollen Ole e I family allergens conf_class=HC
Medtr6g042490 Mt4.0v1 chr6 14674127-14675282 known SSP LAT52/POE32 0.873 255 pollen Ole e I family allergens conf_class=HC
Medtr7g016950 Mt4.0v1 chr7 5358846-5361469 known SSP LAT52/POE2 0.858 465 pollen protein Ole E I-like protein conf_class=HC
Medtr7g027020 Mt4.0v1 chr7 9138594-9140162 known SSP LAT52/POE11 0.684 173 RALF related conf_class=HC
Medtr7g102770 Mt4.0v1 chr7 41638409-41639427 known SSP LAT52/POE16 0.739 182 pollen Ole e I family allergens conf_class=HC
Medtr7g405770 Mt4.0v1 chr7 248231-249512 known SSP LAT52/POE12 0.742 173 Lipid transfer protein conf_class=HC
Medtr8g092230 Mt4.0v1 chr8 38541582-38542715 known SSP LAT52/POE36 0.849 296 pollen protein Ole E I-like protein conf_class=LC
MT4Noble_011084 Mt4.0v1 chr2 24429027-24433758 known SSP LAT52/POE28 0.667 114 Pollen Ole e I family allergen
MT4Noble_024486 Mt4.0v1 chr4 19117221-19118595 known SSP LAT52/POE8 0.776 139 Pollen Ole e I family allergen